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Chromatography Types That Are Often used in Reagent Analysis

By Viola J Wolfson / January 3, 2019

In the chemical reaction industry, one of the reactivos quimicos which are often used chromatography. Chromatography is a method of separating components in a mixture, liquid or gas, in the same plane such as distillation, crystallization, or fractionation extraction. Chromatography is widely applied because many heterogeneous mixtures, or in solid form, can be broken down by suitable solvents. Chromatography can also be said as a method of physical separation, where the separated components are distributed between two phases, one of which is a stationary layer with a wide surface, the other as a fluid that flows gently along the stationary bed. The stationary phase can be either solid or liquid, while the mobile phase can be either liquid or gas.

There are several ways to classify chromatographic techniques; the most important is based on the type of phase used:

– Liquid-Solid Chromatography (LSC)
LSC is absorption chromatography. As an adsorbent silica gel, alumina, molecular filter or porous glass are used in a column where the mixed components are separated by the mobile phase. Column chromatography and thin layer chromatography (TLC) are separation techniques that enter this group.

– Solid Gas Chromatography
It was used before 1800 to purify gas but later did not become famous. Hesse, Claesson, and Phillips were the main contributors in 1940. In the past, this technique had the same disadvantages as liquid adsorption chromatography. But recent research with a new type of solid phase extends to the application of this technique.

– Gas-Liquid Chromatography
Maybe this is the best and versatile technique of all separation techniques. This caused a revolution in organic chemistry since it was first introduced by James and Martin in 1952. The sample size of less than micrograms to more than 100 grams can be handled and 10-15 grams of microorganisms can be detected.

That is three of several types of chromatographic techniques that are quite often used in chemical laboratories. The use of the chromatography technique is adjusted to the type of molecule used.

About the author

Viola J Wolfson