Do you know what electroforesis is? If you often go to the laboratory, you will be familiar with terms like this. Well, electroforesis is the process of moving charged molecules in an electric field. The speed of molecules moving in the electric field depends on the charge, shape and size. The position of molecules that are stranded on the gel can be detected by colouring or autoradiography, or even quantification with a densitometer.
Meanwhile, what is meant by electroforesis gel is a technique used in laboratories to separate molecules such as DNA, RNA and proteins based on their size.
Its history can also be said to have existed long ago, wherein 1937, a Swedish scientist named Arne Tiselius developed to measure the movement of protein molecules. This is a U-shaped device that uses aqueous media to separate protein molecules.
In 1940, the electroforesis zone was introduced using solid media (eg gels) and allowed for better colouration or visualization of molecular separation.
Then in 1960, capillary electroforesis was developed to provide a versatile electroforesis technique. This type of electroforesis allows the separation of molecules using aqueous and solid media.
Then, do you know, what are the interesting facts of this electroforesis gel? Here are interesting facts.
– When preparing agarose electroforesis, it is best to sprinkle agarose to regulate room temperature, stir and let stand for at least 1 minute before heating. This allows agarose hydrate first, which minimizes during heating.
– Can preserve DNA in agarose gel for long-term storage by using 70% ethanol.
– DNA migration can occur when too much buffer covers the gel. Slow migration results from gradients reducing the stress in the gel.
– Electroforesing gels that are too hot can cause DNA to change the properties of something in the gel. Can also cause agarose gel to deform. Cool the gel with a small fan during electroforesis.
– Buffer electroforesis can affect DNA resolution. The TAE buffer (Tris-Acetate-EDTA) provides better fragment resolution greater than 4 kb, while the TBE (Tris-Borat-EDTA) buffer provides better resolution of 0.1-3-kb fragments. Also, using TBE buffer when electroforesing is greater than 150 V and using TAE buffer with supercoiled DNA for best results.